Bagher Seyyed Alipour; Moghaddeseh Fazeli; Salman Ahmadi Asb Chin; Hamid Cheshomi; Leila Saadat Aldaghi
Volume 22, Issue 5 , November and December 2015, , Pages 919-926
Abstract
Background and objective: Chalcones are belonged to an important group of flavonoids which has proven anti-inflammatory and antineoplastic effects. Assessing cellular Cytotoxicity effect of new synthetic chalcone on HeLa cell line is the aim of present study. Materials and Methods: In this experimental ...
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Background and objective: Chalcones are belonged to an important group of flavonoids which has proven anti-inflammatory and antineoplastic effects. Assessing cellular Cytotoxicity effect of new synthetic chalcone on HeLa cell line is the aim of present study. Materials and Methods: In this experimental in vitro study, HeLa cells were treated with various concentrations of chalcone for 72 hours and cell death rates was evaluated by MTT test. Results were analyzed by Student T-Test and morphological changes were assessed by Acridine orange and propidium iodide (PI) staining. Results: Our results showed that new synthetic chalcone in studied concentrations inhibited tumor cell growth. Highest percent (50.44%) of growth inhibition was seen in 40 µg/ml concentration and after 72 hours IC50 was 40µg/ml. morphological changes of HeLa cells after 24 hours were also strengthened the probability of cellular apoptosis. Conclusion: The results showed that new chalcone has inhibitive effects on HeLa tumor cell line and a probable mechanism of apoptosis can causes cellular death.